World Science and Research PublishingGlobal Journal of Animal Scientific Research2345-43772420140817Effects of Feeding Rumen-Protected Choline and Vitamin E on Serum Protein Fractions, Total Thiol Molecules and Total Antioxidant Capacity in Early Lactating Dairy Cows337441ENMohammadrezaRahmaniDepartment of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj. m.rahmani2002@gmail.comMehdiDehghan-banadakyRomicKamalyanHassanMalekinejadFatemehRahmaniMohammad HosseinHadi TavatoriHamedMohammadi2014073020140817Twenty four primiparous and multiparous Holstein cows on early lactation, beginning five weeks postpartum, were used for four weeks to investigate the effects of supplementation of rumen-protected choline (RPC) or vitamin E on blood serum protein fractions, plasma total thiol molecules (TTM), and plasma total antioxidant capacity (TAC). Cows were randomly assigned to one of the following treatments: I - no supplement (control), II - 90 g/d of RPC, and III - 4400 IU/d of vitamin E. Serum protein electrophoresis of samples exhibited four main fractions in the blood serum of the cows including: albumin, α, β, and γ. The electrophoresis was carried out by capillary zone electrophoresis (CZE). In this study, feeding RPC or vitamin E affected the blood serum albumin fraction as well as blood plasma TTM (P<0.05) but the treatments did not affect the different fractions of globulin as well as plasma TAC (P>0.05). The results showed that the increases in serum albumin fractions and TTM which observed in this study, pointed towards a beneficial role of RPC and vitamin E in early lactating dairy cows.