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Email this article Evaluation of effectiveness of three commonly used disinfectant on clinical isolate of Pseudomonas aeruginosa
Abstract
This research was designed to evaluate the effectiveness of three commonly used disinfectants on clinical isolate of Pseudomonas aeruginosa using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Pseudomonas aeruginosa. Isolates of Pseudomonas aeruginosa were collected from Ondo State Trauma and Surgical Center, Ondo. The identity of the isolate was confirmed using cultural, morphological and biochemical characteristics. Three major disinfectants namely Jik(Sodium Hypochlorite), Dettol(Chloroxylenol) and Izal were evaluated on their effect on Pseudomonas aeruginosa. The effect of percentage weight per volume (%w/v) of the chemical composition of each of the disinfectants were determined on the isolate of Pseudomonas aeruginosa using MIC and MBC. A serial concentration of 0.125%w/v to 35%w/v of the chemical composition of the disinfectant was made. The antibiotic susceptibility of the isolate was determined by discs diffusion method as well. The minimum inhibitory concentration MIC of Jik was found to be 0.5%w/v and MBC was 1.0%w/v while the MIC and MBC of Dettol was 1.5 and 2.0%w/v respectively. Izal had MIC of 5% w/v and the MBC of 10.0%w/v. Izal showed the lowest activity based on the chemical composition in %w/v while Jik showed the highest activity. The antibiotic susceptibility test showed that the isolate had multiple resistance by resisting seven out of the ten antibiotic used. The resuls were compared with in use dilution as recommended by the manufacturer of disinfectants. The three disinfectants are effective if used as directed with Jik as the most effective at 0.5v/w% MIC.
Keywords: Antibiotics, Clinical, Composition, Disinfectant, Inhibitory.
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