Determining of Degradation Parameters of Sorghum Silage with Different Levels of Fibrolytic Enzymes Using in Situ Technique

Ghassem Jalilvand, Faezeh Fazeltorshizi, Majid Salari, Mehdi Moghaddam


This experiment was conducted to determine of nutritive value, chemical composition and digestibility sorghum bicolor silage with different levels of fibrolytic enzymes using in situ technique. Chemical compositions were measured according to the standard methods. Three fistulated-Baluchi male lambs used factorial experiment in a completely randomized design. Treatments were A: sorghum silage, B: sorghum + 3g fibrolytic enzymes before silage, C: sorghum + 6g fibrolytic enzymes before silage, D: sorghum + 9g fibrolytic enzymes before silage, E: sorghum + 3g fibrolytic enzymes after silage, F: sorghum + 6g fibrolytic enzymes after silage and G: sorghum + 9g fibrolytic enzymes after silage. The amounts of degradation were measured using nylon bags at 3, 6, 9, 12, 24, 36, 48, 72 and 96 h times. The degradability parameters of dry matter for potential degradability fractions (a+b) were 60.77, 61.52, 64.43, 68.56, 62.60, 63.04 and 66.95% for treatments, respectively. The results showed significantly differences in degradability of experimental treatments in different incubation times and treatment D had highest and treatment A had lowest rumen degradability of 96 h. The result showed that enzymes were causes the significant reduction of NDF and ADF and significant increase of CP, EE and WSC in silage. Between the enzymes is added before or after the silage process can be concluded that in both cases, the enzyme causes significant changes in the treatments containing silage compared to the control treatment. So, this process for feeds in the local animals are benefit and nutrition value of them is suitable, therefore we can recommend them for providing part of roughage requirements in animal feed.


Degradability, Enzyme, In situ, Nutritive Value, Sorghum

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